Optical technologies have become an essential tool in neuroscience to record the structure and function of neural circuits in-vivo and the past years have seen a remarkable rate of innovation and development which has continually raised their performance. However, optical approaches also face omnipresent trade-offs between field of view, acquisition speed, imaging depth, and tissue irradiation, which can be difficult to comprehend from the user perspective. In this workshop, we aim to showcase and discuss the latest strategies that enable neural recordings across scales, from synapses and dendrites to brain-wide circuits, from sub-second dynamics to developmental timescales. Specifically, the presentations will cover: *) Whole-brain imaging in transparent model organisms using light-sheet approaches *) Cortex-wide multiphoton imaging using temporal multiplexing or random-access scanning *) Deep-tissue functional and structural microscopy using adaptive optics In addition, we plan to stimulate discussions on the tradeoffs involved in these different approaches and foster an open exchange of current challenges and future directions. To catalyze this, our workshop will also include a dedicated and moderated 30min discussion section with the panelists and expert users in the audience. This workshop will provide a multidisciplinary perspective on the current state-of-the-art in neuroimaging strategies that could catalyze new research in circuit and systems neuroscience.
Moderators:
-Emmanuel Beaurepaire, Ecole Polytechnique, France
-Robert Prevedel, European Molecular Biology Laboratory, Germany
Speakers:
-Robert Prevedel, European Molecular Biology Laboratory, Germany – High resolution deep brain imaging using adaptive optics three-photon microscopy
-Elizabeth Hillman, Zuckerman Mind Brain Behavior Institute – Light sheet microscopy for volumetric imaging in neuroscience
-Alipasha Vaziri, Rockefeller University, USA – High-speed cortex-wide brain imaging with multiplexed multi-photon microscopy
-Angus Silver, University College London, UK – Multiscale 3D imaging of neural circuits with nonlinear acousto-optic lens microscopy
-Benjamin Judkewitz, Charite, Germany – Whole brain imaging in the smallest vertebrate brain.
Course Features
- Lectures 0
- Quizzes 0
- Duration 50 hours
- Language English
- Students 138
- Assessments Yes
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